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      Urgent! Molecular and Cellular Biologist DAF Functional Genomics - Local Job Opening in Cambridge
 
                        
                         Description: Description
   POSITION SUMMARY: We are seeking a scientist to join our Systems Immunology Lab in *** s Inflammation & Immunology Research Unit.
The successful candidate will be responsible for generating massive single-cell perturbational data using functional genomics and single cell transcriptomics technologies in collaboration with a small team.
   
   POSITION RESPONSIBILITIES: Generate custom lentiviral libraries and maintain extensive cell cultures.
Generate edited pools of cells via lentiviral transduction and arrayed electroporation.
Generate single-cell data via several commercial workflows, including from 10x Genomics.
Develop, modify, and customize such procedures as needed to succeed in specific cell lines and types, and at large scale.
   
   ORGANIZATIONAL RELATIONSHIPS: Reports to a lead scientist in Systems Immunology Lab.
Collaborates with and supports several multi-disciplinary experimental teams on a per-project basis.
   
   EDUCATION AND EXPERIENCE: Bachelors or Masters + 3 years of experience in Biology, Immunology or a related field 
   
   TECHNICAL SKILLS REQUIREMENTS: 3+ years of hands-on experience with standard cell culture techniques including primary immune cells handling and isolation, maintenance of cell lines and in vitro stimulation of primary immune cells, experience with standard molecular biology techniques including lentivirus infection, qPCR, western blot, and flow cytometry.
Bonus: direct experience in pooled cell screening of immune cells using CRISPRi, CRISPRa, and other methods of in vitro gene editing.
   
   PHYSICAL POSITION REQUIREMENTS:  Typical bench work, including sitting, pipetting, lifting <10lb items
   
   Hard Skills:
   1.
Diverse Cell Types
   2.
Molecular biology techniques *qPCR
   3.
 Gene Editing
   
   Preferred:
   Direct experience in pooled cell screening of immune cells using CRISPRi, CRISPRa, and other methods of in vitro gene editing.
   
   Interview Process: 
   1st Teams screen (Camera-On) 
   2nd Onsite (2hr with Stakeholders) 
   
   Duration: 6-months 
   
   Custom Fields: 
   Name: Minimum Education
   Value: Bachelors or Masters + 3 years of experience in Biology, Immunology or a related field
   
   Name: Hours of overtime
   Value: None
   
   Name: WBS Element
   Value: None
   
   Name: Work Environment
   Value: Onsite
   
   Name: Will overtime be needed?
   Value: No
   
   Name: Special Clearances / Medical Surveillance
   Value: {items=[{itemValueId=0280a31e-4678-47d2-a846-0862830ff345, itemValue=Lentivirus}, {itemValueId=8922102d-d2e3-4a25-b77c-700b4f93eaf2, itemValue=Unscreened blood, human tissue or human cell lines}]}
   
   Name: GL Account
   Value: 41160000
   
   Name: Department
   Value: R&D - I&I  |  PTx Clinical Research (815137)
   
   Name: Is this position a Sunshine Act position?
   Value: No
   
                      
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Unlock Your Molecular and Potential: Insight & Career Growth Guide
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Great news! Aequor Technologies LLC is currently hiring and seeking a Molecular and Cellular Biologist DAF Functional Genomics to join their team. Feel free to download the job details.
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An organization's rules and standards set how people should be treated in the office and how different situations should be handled. The work culture at Aequor Technologies LLC adheres to the cultural norms as outlined by Expertini.
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